Mi-20a is a small non-coding RNA downregulated in liver sinusoidal endothelial cells during liver metastasis.
Span-based nanoparticles loaded with miR-20a and decorated with chondroitin sulfate can introduce the missing miR-20a specifically in the liver sinusoidal endothelial cells blocking the angiogenic process.
Anti Cancer Stem Cells therapy
Cancer Stem Cells have different protein expression profile compared with differentiated cancer cells. Identification of critical proteins related with the differentiation process is crucial. Span-based nanoparticles can be decorated with nanobodies against CSC marker CD133 to lead differentiation proteins mRNA.
Computational Biology is a strong tool to find and select immunogenic epitopes. The epitope’s mRNA is included in span-based nanoparticles to lead to CSC and produce immune response